FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP1_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP1_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4087024
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG41490.10151640900567258TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT41080.10051323407936924TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG30000.029.51644142
AATCGGG36150.026.04406241
TCGGGGG25250.025.78197943
CGGGGGT13800.025.36212344
GAATCGG39750.023.77340140
TCCGGAG52250.022.4400231
ACTCCGG52800.022.13998229
CTCCGGA54150.021.84655430
AGAATCG45750.021.72660839
CACTCCG54850.021.63155628
CCGGAGA53900.021.42840632
CGGAGAA54600.020.64086533
TCGGGGT9950.018.9948343
GGAGAAT58300.018.61049334
GTCACTC64500.018.50373526
TCACTCC64900.018.3357627
CAGTCAC68350.017.4614624
GAGAATC58350.017.4548838
AGTCACT70650.017.09116225
CCAGTCA73000.016.30124723
Produced by FastQC (version 0.11.5)