FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP11_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP11_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15079447
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT168830.1119603391291471TruSeq Adapter, Index 7 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC159580.10582616192755609TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66900.025.32104348
ATGCCGT70100.023.86559749
CGTATGC74700.023.05190846
GCCGTCT68800.022.89214151
GTATGCC75150.022.77415547
TGCCGTC73750.022.68444850
TCGTATG83250.020.51624745
CTCGTAT76900.019.88920644
CCGTCTT94250.016.78492552
TCTCGTA89350.015.74685643
CTGCTTG93550.015.339190559
CTTCTGC115300.013.62948456
CATCTCG100550.013.40111841
ATCTCGT103850.013.31229642
CGTCTTC120150.013.10844253
GCTTGAA119500.012.88686161
CACGAGA142200.012.45412731
CGAGATT147350.012.35138333
TGCTTGA123050.012.20219760
TCACGAG148200.012.1625430
Produced by FastQC (version 0.11.5)