FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP25..ps-bZIP11_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP25..ps-bZIP11_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12928503
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT152040.11760062243865357TruSeq Adapter, Index 7 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC146430.11326137295246015TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG65450.024.75909248
ATGCCGT68000.023.31592449
CGTATGC71900.022.87875246
GCCGTCT65250.022.7430551
GTATGCC72500.022.64113447
TGCCGTC71050.022.11798950
CTCGTAT73300.020.29309544
TCGTATG81150.019.96897745
CCGTCTT84650.017.90293752
TCTCGTA84350.016.38986443
CTGCTTG87400.015.49754459
CATCTCG94100.014.28252341
CGTCTTC108700.013.74870153
ATCTCGT99200.013.54824142
CTTCTGC105200.013.24130256
CACGAGA131950.013.10335831
GCTTGAA111450.012.90699361
TCACGAG137800.012.72492430
GTCACGA140100.012.46605629
CGAGATT139150.012.42535433
Produced by FastQC (version 0.11.5)