FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP53_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP53_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379217
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT75040.17135483352389252TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC56300.12856179540771787TruSeq Adapter, Index 7 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC19200.030.62460550
TATGCCG20650.030.3385948
GCCGTCT18000.029.36073351
ATGCCGT20300.029.30996749
CGTATGC22350.028.81394646
GTATGCC23150.027.969447
TCGTATC35800.023.8544438
CGTATCT31650.022.78011739
CCGTCTT24500.022.57113652
CTCGTAT27050.022.25479744
TCGTATG27950.021.28773945
TATCTCG32450.021.13994241
TCTCGTA30050.020.38243343
TTCGTAT48200.019.09726537
ATTCGTA49150.019.01298136
GTATCTC36700.018.88259140
CACGAAT53350.018.82824931
ATCTCGT35950.018.59503242
TATCGCG6400.018.5935141
GTCACGA54300.018.30547329
Produced by FastQC (version 0.11.5)