FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP53_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP53_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6574631
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT196070.2982220599148454TruSeq Adapter, Index 7 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATGCC161980.24637124121490622TruSeq Adapter, Index 7 (96% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66630.10134409064174096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC62000.029.4674644
CGTATGC60750.029.32482143
TATGCCG60300.028.96323845
ATGCCGT56250.028.31083946
GCCGTCT45800.027.81632848
TGCCGTC54750.027.74402647
CTCGTAT64450.026.33799641
TCGTATG67450.025.2703342
AAGGGGG15550.025.20876170
TCGTATC91350.024.3677735
CGTATCT77500.023.89027636
TCTCGTA69350.023.61909540
TATCTCG74550.023.28616338
CCGTCTT58000.022.87047249
ATCTCGT75700.022.51629639
ATTCGTA116300.022.0893833
GTATCTC81750.021.53496237
ATGCCGG10950.021.41532146
TTCGTAT113700.021.36319734
CGTATCG19550.020.94625336
Produced by FastQC (version 0.11.5)