FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP44_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP44_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8597885
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT120140.1397320387513906TruSeq Adapter, Index 13 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATGCC99740.11600527338990928TruSeq Adapter, Index 13 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43850.025.30192448
ATGCCGT45500.024.0766949
GTATGCC47900.023.74716447
TGCCGTC45550.023.7429150
CGTATGC47850.023.33310946
GCCGTCT39300.023.06593551
TCGTATG50500.020.5839645
CTCGTAT47550.019.72641444
CATTCAG92150.017.31942233
CCGTCTT53850.017.15870952
TCTCGTA58700.016.09865243
CTGCTTG49800.014.96973559
CACATTC113750.014.09217231
TTCAGAA117000.013.82037935
TGGGGGG13050.013.67826751
ACATTCA119700.013.50864232
CAGAAAT115350.013.47191137
AATCTCG77500.013.45793541
GCTTGAA62750.013.44210161
ATTCAGA120550.013.29725934
Produced by FastQC (version 0.11.5)