FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP44_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP44_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7908200
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT93990.11885131888419616TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35150.023.89751848
GTATGCC39150.021.9027447
ATGCCGT36500.021.86295349
CGTATGC39550.021.50423246
TGCCGTC37850.019.78858650
TCGTATG41550.019.37407345
GCCGTCT33950.019.27829748
CTCGTAT40650.018.68371844
CATTCAG74500.016.72468233
CCGTCTT40700.016.51101149
AAGGGGG11450.014.36668270
TCTCGTA53150.013.96036243
CACATTC91300.013.80053228
CTCCAGT94700.013.34201321
CTGCTTG43700.013.29507456
ACTCCAG93800.013.24623220
ACATTCA98500.012.93389329
TTCAGAA99600.012.79104935
GCTTGAA54050.012.56232858
ATTCAGA101300.012.40363931
Produced by FastQC (version 0.11.5)