FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP2_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP2_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080548
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT95330.3094579276154762TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC84650.27478877134847435TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26400.030.22687348
GTATGCC27000.029.68479347
CGTATGC27550.029.09217546
ATGCCGT26950.028.96065549
GCCGTCT25850.028.83907751
TGCCGTC27150.028.48949250
TCGTATG30600.025.84932945
CTCGTAT28900.025.4321944
CCGTCTT29900.024.81572352
TATCTCG30750.024.58504141
CTGCTTG28000.024.12468159
CGCTCAT34400.024.0113134
ATCTCGT32700.023.22599442
TCTCGTA31600.023.03767243
CCGCTCA36800.022.82578733
CGTCTTC33500.022.04448553
ACCGCTC37250.021.98628629
CACCGCT38200.021.43950828
GCTCATT40700.021.06851835
TCACCGC39550.020.53070330
Produced by FastQC (version 0.11.5)