FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP2_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP2_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516821
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT111120.3159671760376772TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC102690.29199666403265906TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC30950.030.30671150
TATGCCG31650.030.29992148
CGTATGC32750.030.24404146
GCCGTCT29350.029.81237851
ATGCCGT32500.029.076749
GTATGCC33750.028.93311147
CTCGTAT35050.027.360744
TCGTATG36800.026.82044445
CCGTCTT34100.026.37809652
TCTCGTA37300.025.52259343
TATCTCG38900.024.7427441
CTGCTTG33450.024.06558459
ATCTCGT40150.023.88524442
CGCTCAT45000.023.41092934
CCGCTCA45600.022.8726333
ACCGCTC46800.022.28615232
CACCGCT48350.021.35454231
CGTCTTC41650.021.17630653
GCTCATT50400.020.7637335
CTTCTGC40350.020.64420156
Produced by FastQC (version 0.11.5)