FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP1_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP1_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7097380
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG45400.028.52380842
TCGGGGG39100.025.33210643
AATCGGG56550.025.12783441
CGGGGGT22950.023.48549344
GAATCGG65950.022.23621440
TCCGGAG85050.022.1395934
CACTCCG85500.021.7365231
ACTCCGG86900.021.30578432
CTCCGGA90800.020.6219433
AGAATCG77000.019.95425239
TCACTCC101300.018.41532330
CCGGAGA101150.018.40803335
CGGAGAA99300.018.36327436
TCGGGGT16550.017.97556543
CGGGGGG28700.017.68266744
GTCACTC108550.017.41107429
CAGTCAC111700.016.9200727
AGTCACT119500.015.96210828
TCCAGTC120300.015.68139725
CCAGTCA120850.015.46522426
Produced by FastQC (version 0.11.5)