FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP1_Col..h-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP1_Col..h-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5433600
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT55580.1022894581861013TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG40250.030.52181842
AATCGGG47350.026.83217241
TCGGGGG34600.026.4015643
CGGGGGT18550.024.90572741
GAATCGG54850.023.8013440
TCCGGAG73700.023.60228734
CACTCCG74000.023.31741531
ACTCCGG74450.023.27049832
CTCCGGA75850.022.84098233
TCGGGGT14350.021.95107743
AGAATCG64850.021.1565739
CCGGAGA85250.020.24033535
CGGGGGG24950.019.77942844
CGGAGAA84600.019.77546736
TCACTCC87500.019.7598730
GTCACTC89700.019.50934829
CGGGGTT12750.018.39203644
CAGTCAC96600.018.18828627
AGTCACT101250.017.3875428
TCCAGTC102900.017.04070525
Produced by FastQC (version 0.11.5)