FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ph-bZIP10..ps-bZIP11_Col..h-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ph-bZIP10..ps-bZIP11_Col..h-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8896878
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23350.019.78559345
ATGCCGT25750.018.62110146
TGCCGTC26850.018.11892947
GCCGTCT26850.017.85822548
GTATGCC27800.017.24796144
CGTATGC28650.016.1254343
CTCGTAT30000.014.34980141
TCGTATG32800.013.97846442
CCGTCTT41500.012.1444149
CACGAGA44400.011.50884928
TCACGAG45600.010.89897227
TCTCGTA37050.010.58020940
CTCCAGT51050.010.14677421
AGTCACG53000.09.9715625
GTCACGA50900.09.97039226
ACTCCAG54100.09.63958520
CTGCTTG41800.09.629106556
CGAGATT55000.09.48168730
GAACTCC56450.09.362926518
CTTCTGC50050.09.02090553
Produced by FastQC (version 0.11.5)