FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP63_Col-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP63_Col-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27124841
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT329520.12148273975135927TruSeq Adapter, Index 19 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATGCC305390.11258683507121756TruSeq Adapter, Index 19 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG108950.026.05304148
TGCCGTC112100.024.69651650
GCCGTCT114100.023.2513651
GTATGCC122700.023.13348847
ATGCCGT126800.021.86103649
CGTATGC162600.017.6720746
GCTATCT149700.017.1842139
CTCGTAT179800.015.68950944
CCGTCTT179150.015.4338552
TCGTATG195000.014.86142845
AGCTATC178550.014.78003738
CTGCTTG184600.013.669970559
ATCTCGT195350.013.5627142
CTATCTC199800.013.3131940
CTGAAGC230450.013.16759334
CGTCTTC211550.012.90463253
AAGGGGG34200.012.89461870
GAAGCTA241600.012.64681936
TATCTCG208150.012.52690741
CCTGAAG241550.012.47556133
Produced by FastQC (version 0.11.5)