FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP53_Col-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP53_Col-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10073662
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAT93450.026.59154537
GCGCATC86650.026.49718738
ATGCGCA102300.025.9333236
AATGCGC108100.025.31894535
TATGCCG29750.023.64686845
TAATGCG119250.023.06899534
CATCGCG28900.022.76798238
CGCATCG52750.021.76285736
GTATGCC35150.020.41235444
CTAATGC138150.020.14098733
CGCATCT62400.019.68734239
TGCCGGC15950.019.31018850
GCATCGC35950.019.27661937
ATGCCGT29800.019.02669149
CTCGTAT36550.018.86441644
CGTATGC32950.018.69484143
ATGCCGG16850.018.27878446
CATCGGG24600.018.21123541
GCATCGG25100.018.12734440
CATCTCG53100.017.6646441
Produced by FastQC (version 0.11.5)