FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP53_Col-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP53_Col-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5742945
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC53150.028.4477138
TGCGCAT58100.027.95172737
ATGCGCA63200.027.35751736
AATGCGC66200.027.1751535
TATGCCG18500.025.54024348
TAATGCG70750.025.4769634
CATCGCG19250.025.09083741
CGCATCG32250.023.98442839
GTATGCC21300.023.00442947
TGCCGGC9850.022.38552950
CGTATGC19450.022.31336646
CTAATGC82150.021.94150733
ATGCCGG10250.021.85340549
CGCGTAT11250.021.77771644
GCATCGC23500.021.44674740
ATGCCGT18400.021.11388649
ATCGCGT14900.020.43618242
CGCATCT37750.020.30457739
CATCGGG12300.019.91864241
TCGCGTA12000.019.83327743
Produced by FastQC (version 0.11.5)