FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP44_Col-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP44_Col-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10187096
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC180620.17730273671711744TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT178520.17524130527483006TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG68450.026.63965848
ATGCCGT71250.025.39627649
CGTATGC73100.025.23234643
GCCGTCT70750.024.73477451
GTATGCC75050.024.4834744
TGCCGTC74550.024.22514750
CTCGTAT71700.022.7961841
TCGTATG82550.022.25904842
CCGTCTT87100.020.29259552
TCTCGTA80800.018.8426643
GATCTCG77700.018.7385841
CTGCTTG89650.017.99761659
CGATCTC84300.017.47909440
TTACTCG113300.017.29905932
CTTCTGC102150.016.7546356
GCTTGAA100950.016.6070961
CGTCTTC107900.016.2510253
ATCTCGT95600.015.37643842
ATTACTC127450.015.35098731
TGCTTGA107150.015.1235360
Produced by FastQC (version 0.11.5)