FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP44_Col-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP44_Col-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11829783
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC252730.21363874552897547TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT243830.20611536154128948TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG92650.028.10538745
ATGCCGT95200.027.27903446
GTATGCC97700.026.83176844
GCCGTCT92150.026.62469948
TGCCGTC96300.026.4949647
CGTATGC98400.026.4274843
TCGTATG105250.024.17543642
CTCGTAT95800.024.002841
CCGTCTT109400.022.90644849
CTGCTTG111600.019.91459856
TCTCGTA105950.019.8534340
GATCTCG101650.019.83248538
CGATCTC108350.018.92913237
CGTCTTC134950.018.1546250
GCTTGAA128350.018.0519558
TTACTCG153000.017.82001532
CTTCTGC127800.017.7736253
ATCTCGT116600.017.52977439
TGCTTGA133100.017.09217557
AAGGGGG23500.016.23381870
Produced by FastQC (version 0.11.5)