FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP2_Col-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP2_Col-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7266105
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27600.024.34758845
ATGCCGT28050.024.2065446
TGCCGTC28550.023.41483547
GCCGTCT29100.022.4911948
CGTATGC30000.022.1664543
GTATGCC31400.021.51252744
TCGTATG34150.019.78018842
CTCGTAT32700.017.76741241
CCGTCTT39850.016.77524449
TTACTCG46000.015.52158732
GATCTCG35050.015.47773738
CTGCTTG40500.015.12330956
TCTCGTA37650.014.22296940
CGATCTC39750.013.119369537
TACTCGA55050.012.77916733
ATTACTC57100.012.50425731
CTTCTGC52250.012.32523953
CGTCTTC55450.012.18202850
GCTTGAA52200.011.9347558
CAGTCAC61600.011.93170324
Produced by FastQC (version 0.11.5)