FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP2_Col-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP2_Col-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6757057
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT79200.11721079162126352TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC75390.11157224217584667TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30600.025.73496648
GTATGCC33300.023.64834447
ATGCCGT33650.023.61039749
GCCGTCT33450.023.43766651
CGTATGC34300.023.0609346
TGCCGTC34550.022.7927650
TCGTATG38000.020.262945
CTCGTAT36350.019.44954544
CCGTCTT42250.019.05300952
TTACTCG47750.017.518135
CTGCTTG43500.016.49404359
GATCTCG38050.016.46496641
TCTCGTA40200.016.19382343
CGATCTC40950.015.04254240
CGTCTTC56100.014.28680553
TACTCGA56050.014.17466336
CTTCTGC53800.013.92175556
ATTACTC62200.013.78599734
GCTTGAA55000.013.49073661
CTCGATC49000.013.21411638
Produced by FastQC (version 0.11.5)