FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP1_Col-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP1_Col-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4311452
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT15700.022.06982849
TATGCCG16500.020.99977748
GCCGTCT15800.020.60104851
GTATGCC17550.019.7433847
TGCCGTC17300.019.6240750
CGTATGC18250.019.17787746
TCGTATG20300.017.24119645
CTCGTAT18450.016.6935944
CCGTCTT21800.015.09158252
TTACTCG27250.013.357656535
CTGCTTG22700.013.10558859
GATCTCG20150.013.02715741
TCTCGTA23950.012.12930843
CGATCTC22950.011.74279440
TACTCGA32050.011.46633736
CTTCTGC29950.011.21857856
CGTCTTC30150.011.1441653
ATTACTC34700.011.09498334
ACTCGAT28900.010.89953837
GCTTGAA29250.010.76911561
Produced by FastQC (version 0.11.5)