FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP1_Col-B_p1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP1_Col-B_p1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8497165
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31500.021.444348
GCCGTCT30400.020.95380651
TGCCGTC32550.020.53749350
GTATGCC33200.020.45166847
ATGCCGT33950.020.10295749
CGTATGC35050.019.7716246
TCGTATG40350.016.56742142
CTCGTAT38400.015.85926741
CCGTCTT40850.015.42217252
CTGCTTG43950.013.93619559
TTACTCG52200.013.61101832
GATCTCG40900.013.17839541
TCTCGTA43900.012.75617840
CGATCTC43700.012.574285540
GCTTGAA58850.011.77562158
CTTCTGC56100.010.98031756
TACTCGA62650.010.83791536
ATTACTC68600.010.61217231
TGCTTGA60100.010.59892960
CGTCTTC60500.010.47100353
Produced by FastQC (version 0.11.5)