FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_bZIP11_Col-B_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_bZIP11_Col-B_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11718856
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30900.019.7085945
ATGCCGT35900.016.76867546
TGCCGTC36600.016.1610847
GTATGCC38100.015.98413144
CGTATGC38350.015.87993243
GCCGTCT37900.015.69909148
TCGTATG46050.013.07264942
CTCGTAT43000.012.77897441
CCGTCTT54150.011.37572249
GATCTCG49950.010.51043138
TCTCGTA56400.09.74283340
TTACTCG71200.09.24150332
CTGCTTG61100.09.10798956
CGATCTC59550.08.81605437
CTTCTGC78900.07.98476553
GCTTGAA76800.07.97523458
ATTACTC86550.07.68336231
TGGGGGG11555.0931703E-117.57595871
TGCTTGA81600.07.50610357
CGTCTTC80750.07.45505250
Produced by FastQC (version 0.11.5)