FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ColSPL9E169G_Col-B_p.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ColSPL9E169G_Col-B_p.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589288
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC36600.025.5325935
TGCGCAT41300.024.9998137
ATGCGCA44750.024.87131736
TATGCCG14250.024.0699945
AATGCGC48850.023.85856835
TAATGCG52800.022.33884634
ATGCCGT13600.022.13218549
CGCATCG20800.022.04310236
GTATGCC15900.021.79228644
CATCGCG12800.021.32796341
CGTATGC14400.020.65956546
TGCCGTC12950.020.54038450
CATCGGG9450.018.88874438
CTGCGTG6000.018.66652556
CTAATGC65200.018.19771433
GCCGTCT10250.018.09742251
GCATCGC15050.017.90684140
GCATCGG10500.017.66653337
CGCATCT28150.017.5309539
ATCGCGT10850.017.41922242
Produced by FastQC (version 0.11.5)