FastQCFastQC Report
Thu 11 Jun 2020
HCCY2BGXF_n01_ColSPL16_Col-B_p2xDna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCY2BGXF_n01_ColSPL16_Col-B_p2xDna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5642244
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC46850.027.5665538
TGCGCAT52350.027.01035537
CGCATCG28150.025.73697939
ATGCGCA59550.025.39028536
AATGCGC63350.024.53025235
TATGCCG16350.024.40353448
CATCGCG16150.024.05559241
TAATGCG69050.022.4546234
GTATGCC19300.021.76153847
ATGCCGT15850.021.64025949
CATCGGG13850.021.48002641
GCATCGG14700.021.42845240
CGTATGC17000.021.20576546
TGCCGTC13550.020.92239250
GCCGTCT10450.020.76543451
ATGCCGG7550.020.39723849
ATCGCGT12700.019.8424142
GCATCGC20400.019.73028240
TCGCGTA10300.019.70862843
CGGGGTG5550.019.5494453
Produced by FastQC (version 0.11.5)