FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29048803
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9128993.1426389583075074No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA453220.1560201981472352No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG432250.14880131205406294No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA405130.13946529913814348No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC402990.13872860785348023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA2177200.068.989591
AGGTAGT2446000.068.7992553
GGTAGTA2457800.068.780874
AGTAGGT1494150.068.719217
TAGTAGG1497350.068.653726
GAGGTAG2495350.068.651932
GTTGTAT1100200.068.6377612
GTAGTAG2454950.068.6241765
TAGGTTG1494550.068.599949
CAGGATA982350.068.5528912
GATAGGC457150.068.5030415
TTCAAGT947750.068.449591
TATAGTT846000.068.4362816
GTAGATT602000.068.400788
TTGTATA1151500.068.3416113
AGATTGT601900.068.2964410
GCAGCAC853000.068.292073
TGTATAG1146900.068.209714
TTGGTAC472150.068.17847412
CCAGGAT707600.068.1454411
Produced by FastQC (version 0.11.5)