FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK287_LM3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK287_LM3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11080251
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA570700.5150605342785105No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT554750.5006655535149881No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA468220.4225716547395903No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG288830.26067099021493284No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC240410.21697161914472876No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT153450.138489642518026No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA150280.13562869649794035No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC139970.12632385313292993No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT111810.1009092664056076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1247750.069.617321
GGATAGG636850.069.5046814
GATAGGT176200.069.46297515
AGGTAGT1373700.069.4273453
GGTAGTA1376200.069.4207844
GTTGTAT623250.069.40964512
GTAGGTT854100.069.408898
AGTAGGT854900.069.373227
GAGGTAG1403450.069.320092
CATTATT344100.069.313731
GATAGGC317100.069.2597815
TAGTAGG856000.069.255356
TGGTACG344950.069.21802513
GTAGTAG1378000.069.1878365
AAGTAAT660600.069.1787344
TATTGGC420400.069.1750815
GCAGCAC486300.069.166573
CAGCACG409350.069.160834
TTCAAGT645300.069.158561
TAGGTTG857250.069.145379
Produced by FastQC (version 0.11.5)