FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK263_LDI4_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK263_LDI4_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11532650
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA526060.45614841341755796No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA468140.405925784620187No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG337820.29292486982610244No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC302440.2622467516138962No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT222900.1932773473572856No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC206450.1790134964643859No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT174630.1514222663481507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1322350.069.647451
GGATAGG653150.069.63422414
GATAGGT180850.069.5531915
AGGTAGT1444800.069.4694753
AGTAGGT902150.069.464737
GGTAGTA1447850.069.449184
GTTGTAT654850.069.41099512
GATAGGC311800.069.4033815
GTAGGTT902650.069.403298
TATTGGC469800.069.3948615
TAGTAGG903100.069.371586
GAGGTAG1474450.069.366312
CAGGATA677450.069.3324412
TTCAAGT663550.069.30321
GGTACGC326650.069.25928514
CGTAAAT460800.069.247569
AGATTGT337050.069.24116510
TGGTACG332050.069.2291613
TAGGTTG904650.069.225749
AAGTAAT679900.069.218964
Produced by FastQC (version 0.11.5)