FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK262_LM1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK262_LM1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11577676
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG365170.3154087227868529No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA258010.2228512872531586No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC222960.19257750864681306No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA197050.17019823322055308No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT187970.16235555391254686No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT135000.11660371217850629No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG128710.11117084292218923No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC125200.10813914640554806No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1375850.069.603711
GGATAGG674600.069.5782714
AGGTAGT1507450.069.497123
GGTAGTA1510350.069.491774
GTAGGTT935850.069.450858
GATAGGC328000.069.4329715
GTTGTAT692850.069.42798612
AGTAGGT936900.069.387967
GAGGTAG1541800.069.360872
TAGTAGG938000.069.341586
GATAGGT182950.069.2906815
GCAGCAC514850.069.285283
CATTATT352200.069.28291
GTATGGT340350.069.2786815
TGGTACG353000.069.2648213
CCAGGAT508500.069.2554611
CAGGATA697850.069.25152612
TAGGTTG939150.069.209659
TATTGGC447850.069.209215
TTCAAGT682250.069.187181
Produced by FastQC (version 0.11.5)