FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK261_LP6_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK261_LP6_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10341196
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA446760.4320196619423904No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA423300.40933369795911423No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC420660.4067808017563926No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG261030.2524176120441001No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT240780.23283573776186045No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC154670.1495668392708155No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT135180.13071988965299564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1005850.069.624561
GGATAGG492600.069.5729314
GCAGCAC609450.069.491063
CGTAAAT542650.069.445239
GTTGTAT483500.069.44083412
TATTGGC543050.069.43851515
TGGTACG273050.069.38296513
GTAGGTT660650.069.354258
GAGGTAG1132850.069.344832
CAGCACG539750.069.339164
AGTAGGT661250.069.333677
AGGTAGT1116150.069.282433
GGTACGC267200.069.2788114
AAGTAAT515600.069.266524
TTCAAGT503100.069.25241
TAGCAGC585850.069.246991
GATAGGT153300.069.2458215
TAGTAGG663050.069.228546
GGTAGTA1119350.069.2257844
TTGGCGA430800.069.21117417
Produced by FastQC (version 0.11.5)