FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK256_LDI2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK256_LDI2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10822080
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC337750.31209342381501526No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA300760.2779133031727727No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG285450.26376630000887075No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC153380.14172876193855524No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA151220.13973284248499365No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT139830.12920806351459238No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCG120140.11101377923652385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1108550.069.592331
GGATAGG565150.069.5579114
CGTAAAT610850.069.501389
GATAGGC261550.069.4489615
GTAGGTT719950.069.427188
TATTGGC609500.069.4233815
GCAGCAC685350.069.379153
AGTAGGT720550.069.354797
AGGTAGT1223200.069.327293
GTTGTAT521350.069.3269312
GGTAGTA1225800.069.309424
GTAAATA615000.069.29986610
CAGCACG609550.069.265294
TGGTACG267950.069.2400213
TTGGCGA483850.069.2380917
GTAGATT301450.069.234538
GAGGTAG1242650.069.231122
AAGTAAT590200.069.228564
AAATATT642900.069.2197612
TAGCAGC662300.069.207781
Produced by FastQC (version 0.11.5)