FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK255_LP5_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK255_LP5_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10471415
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC428900.4095912539040808No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA346180.33059524429124426No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG303830.29015180851871497No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA288990.2759798938347874No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT225250.21510941930961577No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC159420.15224303496709854No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT156870.14980783399378214No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG112010.10696739647889039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1154050.069.581431
GGATAGG546750.069.5632514
GTAGGTT780400.069.366188
GGTAGTA1288200.069.363634
AGGTAGT1284950.069.359263
AGTAGGT781400.069.3586967
GATAGGC254550.069.3523215
GATAGGT152750.069.3340715
TGGTACG338200.069.32587413
CAGGATA568300.069.3026212
GTTGTAT574150.069.28532412
TAGTAGG782700.069.271636
GAGGTAG1313350.069.250892
GGTACGC332950.069.2311814
CGTAAAT393450.069.22439
TATTGGC398650.069.2083915
GCAGCAC459200.069.2028353
AAGTAAT571100.069.184894
GTAGTAG1288100.069.173335
TTCAAGT554250.069.1592251
Produced by FastQC (version 0.11.5)