FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK254_LP3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK254_LP3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10552737
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA281590.2668407257756921No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC253370.2400988482893111No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG249640.23656422025868737No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA146990.1392908778073404No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT140650.13328295777673602No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC108610.10292116632869748No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG107920.10226730752410489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1021600.069.622361
GGATAGG508150.069.6124914
CGTAAAT563150.069.5464869
GTAGGTT647250.069.508438
GCAGCAC633750.069.465033
TATTGGC562700.069.4259715
AGTAGGT649000.069.4241267
AGGTAGT1134550.069.3663563
GGTAGTA1138500.069.344674
ATTGGCG501500.069.3212416
GTAAATA566950.069.30810510
GAGGTAG1152900.069.3031852
GTTGTAT477350.069.30231512
CAGCACG562000.069.301724
GATAGGC224550.069.2968115
TAGTAGG650800.069.271026
GATAGGT153150.069.2669115
GAAGTAT343950.069.2560312
TAGCAGC611000.069.2429351
GTATGGT220550.069.2364815
Produced by FastQC (version 0.11.5)