FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK241_LM2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK241_LM2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10591425
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA381060.3597816157882438No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG306270.28916788817368766No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC280510.2648463261553568No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA270800.25567853239767074No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC191160.1804856287043528No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT166080.15680609549706484No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT146590.13840441678055596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1187700.069.652971
GGATAGG595750.069.5704514
AGGTAGT1319850.069.498293
GTAGGTT810550.069.463888
AGTAGGT811800.069.4517757
TAGTAGG813000.069.434866
GGTAGTA1324250.069.4213644
GTTGTAT585750.069.4137512
CGTAAAT445050.069.370519
GATAGGC276050.069.3653815
GCAGCAC505900.069.3304443
GATAGGT166450.069.3054215
GAGGTAG1347250.069.298342
TATTGGC447800.069.2880715
TTCAAGT605650.069.2640841
GTATGGT278000.069.2439315
GTAGTAG1325400.069.2343445
CAGGATA620600.069.2323312
TAGGTTG813050.069.203279
AAGTAAT624250.069.186194
Produced by FastQC (version 0.11.5)