FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK239_LP4_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK239_LP4_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10453099
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA365540.34969533915253265No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC329770.3154758220504752No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA293250.2805388143745697No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG270800.25906192986405274No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT207230.1982474288246959No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC149100.14263712608098325No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT134710.12887087360408622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1141200.069.621621
GGATAGG595400.069.58224514
GTAGGTT761450.069.471688
GGTAGTA1259050.069.471254
AGTAGGT761500.069.467127
AGGTAGT1255800.069.4580153
CGTAAAT410400.069.454139
GATAGGC276750.069.4305115
GTTGTAT556300.069.4019112
GAGGTAG1280800.069.364752
TAGTAGG762850.069.354256
GATAGGT162100.069.3302715
CAGGATA620900.069.2949912
TTCAAGT607100.069.267711
TATTGGC419050.069.2646215
GTAGTAG1260300.069.25795
TGGTACG337900.069.2531713
TTGGTAC338250.069.233912
GGTACGC331050.069.2278314
AAGTAAT624350.069.222794
Produced by FastQC (version 0.11.5)