FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK237_LDI3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK237_LDI3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10551957
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA443130.41995053618963757No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC339740.32196871158591717No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG320160.3034129119366199No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA299400.28373883631254376No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC192650.1825727682552156No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT172980.16393167637055384No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG106480.10091019135123466No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1151200.069.623421
GATAGGT148850.069.59939615
GGATAGG547700.069.5585214
AGGTAGT1271600.069.416213
GGTAGTA1274650.069.394564
GCAGCAC497700.069.369253
GTTGTAT562700.069.33948512
GATAGGC259900.069.33926415
AGTAGGT772700.069.307427
CGTAAAT433300.069.289629
TAGTAGG773200.069.289066
GAGGTAG1296850.069.25212
GTAGGTT772450.069.248638
CAGGATA568850.069.2420112
GGTACGC293850.069.2252614
GTATGGT267100.069.2129115
GAAGTAT372700.069.2099712
TGGAATG384550.069.205491
TATTGGC438100.069.1682715
AAGTAAT572350.069.155584
Produced by FastQC (version 0.11.5)