FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK236_LP1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK236_LP1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10726976
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA480290.4477403510551343No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA372590.3473392687743498No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG287310.2678387646248113No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC270260.25194425716996105No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT177140.16513507627872012No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT135920.12670858963420817No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC129450.12067706686395123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG599350.069.5145314
TGAGGTA1126750.069.504691
GGTAGTA1253500.069.368574
GATAGGT165350.069.36388415
AGGTAGT1251450.069.329853
GATAGGC274350.069.3115
TATTGGC415350.069.30791515
CGTAAAT413550.069.306849
AGTAGGT748350.069.26727
TTCAAGT608550.069.2663961
CAGGATA623800.069.26453412
GTAGGTT747650.069.2618268
TGGTACG318900.069.2529113
GAGGTAG1276000.069.2222
GTTGTAT545200.069.1970912
AAGTAAT627100.069.179124
TAGTAGG749650.069.176246
GCAGCAC475350.069.160913
TTGGTAC319500.069.1341312
CAGCACG412050.069.132914
Produced by FastQC (version 0.11.5)