FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK218_LDI1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK218_LDI1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10239385
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC405100.3956292296851813No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG335900.32804704579425426No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA283710.27707718774125595No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA201020.1963203844762161No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT151920.14836828579060168No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC131110.12804479956559892No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT107960.10543601983908214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1153900.069.6459961
GGATAGG590050.069.6427614
AGGTAGT1280950.069.514243
GTAGGTT785700.069.509688
GGTAGTA1285150.069.496814
GATAGGT162800.069.4823615
AGTAGGT786300.069.46527
GAGGTAG1305750.069.416592
GATAGGC279000.069.4087315
GGTACGC319600.069.4073114
GTTGTAT571950.069.3993112
TGGTACG326450.069.3878913
GCAGCAC480300.069.3565443
TAGGTTG786800.069.3362059
TAGTAGG788000.069.3366
CGTAAAT413850.069.313999
CAGCACG411400.069.305024
TTGGTAC327000.069.3032912
CAGGATA611200.069.2832112
TATTGGC415500.069.2739115
Produced by FastQC (version 0.11.5)