FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_FDK216_LP2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_FDK216_LP2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357274
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC260050.25107957943373904No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA255480.24666722151021592No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA236480.2283226262045399No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG233030.2249916338990356No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT184590.17822257091972268No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT115750.11175720561221031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG546950.069.5898214
TGAGGTA1056500.069.564261
GATAGGT151200.069.5595515
CGTAAAT380050.069.474439
GGTAGTA1178400.069.454444
AGGTAGT1176200.069.438183
TATTGGC381300.069.3843615
GTAGGTT702900.069.3822568
GATAGGC248400.069.379415
GCAGCAC438900.069.329623
AGTAGGT703700.069.31837
TGGTACG263250.069.3076713
GTTGTAT512650.069.2753412
GGTACGC259850.069.2585514
GAGGTAG1200300.069.254022
CAGGATA572150.069.23437512
TTCAAGT558700.069.219141
TAGTAGG705350.069.207256
CAGCACG379250.069.203614
AAGTAAT573700.069.1982354
Produced by FastQC (version 0.11.5)