FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA270_DOM2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA270_DOM2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716964
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG265650.2478780371008058No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA191940.17909922996848734No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA185980.17353795347264395No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT159400.14873615326131542No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT144210.1345623629975803No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC135860.12677097730290032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1284950.069.67271
GGATAGG641300.069.6655614
AGGTAGT1406950.069.4807053
GATAGGT178400.069.46876515
GATAGGC315100.069.4653115
GGTAGTA1411700.069.448454
TATTGGC422500.069.4434515
AGTAGGT862050.069.404567
GTAGGTT861500.069.383228
GAGGTAG1434400.069.370472
GTTGTAT631050.069.3603312
CAGGATA664750.069.34527612
TTCAAGT651200.069.3413241
CGTAAAT412700.069.321319
GGTACGC332400.069.2930114
TAGTAGG864950.069.264096
AAGTAAT668250.069.251184
GTAGTAG1413100.069.243385
TGGTACG338700.069.2334713
AGGATAG664700.069.20306413
Produced by FastQC (version 0.11.5)