FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA269_DOM3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA269_DOM3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10267570
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA357090.34778433456017344No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG287370.27988121824345974No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA274210.26706416415958206No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC268490.26149322575838296No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC179000.17433531010745482No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT162530.15829451369700911No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT143690.13994547882312952No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1149350.069.621971
GGATAGG556400.069.589314
AGTAGGT770300.069.386147
CGTAAAT483900.069.3847359
GTTGTAT565550.069.3796612
GATAGGC269700.069.37527515
GTAGGTT770750.069.368338
AGGTAGT1276900.069.3630143
GCAGCAC558950.069.3606343
GAGGTAG1298800.069.354862
TGGTACG298800.069.3539413
GGTAGTA1280500.069.3272554
TATTGGC493650.069.3246315
GATAGGT158400.069.29111515
GGTACGC294700.069.285614
CAGCACG482100.069.285524
CAGGATA577550.069.23495512
AAGTAAT578950.069.2086644
CCAGGAT411650.069.2078111
TTGGTAC299950.069.18172512
Produced by FastQC (version 0.11.5)