FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA268_DODI2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA268_DODI2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10132392
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG310250.3061962071739822No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC231030.22801131262983115No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA207910.2051934034924823No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT153990.15197793373963425No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA152790.1507936131961732No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC144090.1422072892560809No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT127040.12538006820107236No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT111450.1099937704739414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1076100.069.6272351
GGATAGG509000.069.4072814
CAGCACG510000.069.387474
GTTGTAT518500.069.3715512
TATTGGC517600.069.3562515
AGTAGGT708150.069.353237
AGGTAGT1187800.069.349263
GTAGGTT707650.069.3478248
GGTAGTA1190200.069.333654
CGTAAAT513450.069.32559
GCAGCAC582600.069.285213
GAGGTAG1208150.069.284842
GATAGGT144850.069.22542615
GTATGGT242350.069.2187815
TAGCAGC559650.069.210661
TAGTAGG710000.069.2011646
GTAGTAG1189200.069.186215
AGATTGT281250.069.1786710
GATAGGC255300.069.1760815
AAGTAAT527250.069.175734
Produced by FastQC (version 0.11.5)