FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA267_DODI3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA267_DODI3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10941394
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG477440.43636121686139806No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT360950.329893978774551No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG326190.29812471792899514No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA265670.24281183914956359No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA250050.22853577889618087No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT238820.21827200446305106No Hit
CTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCTA229980.21019259520313407No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACTGCTAATGAAAACACAG184140.16829665397297638No Hit
CCTGCATGATGACGAACAAATACTGACTACCTGAAGATCTTATTAGCTCT155650.14225792435589102No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC152690.13955260179827178No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTA137380.12555986924518028No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT136200.12448139606342666No Hit
GCTTGTGATGAGACATCTCCCACTCATGTTCGAGTTGCTCGACTATGAGA127350.11639284720027449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1247150.069.599681
GGATAGG575200.069.59720614
GATAGGC278450.069.5840115
GTAGGTT860700.069.5133748
AGTAGGT861950.069.461617
CGTAAAT531550.069.433819
GGTAGTA1386250.069.426734
AGGTAGT1383050.069.4070053
TGGTACG248250.069.3781513
TATTGGC535050.069.3773715
TAGTAGG863600.069.3615956
GAGGTAG1410650.069.326482
GGTACGC244650.069.3264214
CAGCACG528600.069.315654
GCAGCAC593700.069.315333
TAGGTTG863150.069.302639
GTAGTAG1386900.069.293535
CAGGATA598000.069.255512
GTATGGT287000.069.24271415
GATAGGT165550.069.2377115
Produced by FastQC (version 0.11.5)