FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA263_DOP3_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA263_DOP3_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9464301
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG261450.2762486104362065No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC242120.25582449247968764No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA206090.21775512000305144No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA188680.1993596780153125No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT151250.1598110626447743No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT144830.15302767737416637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC112730.11911075102112666No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG102170.10795303319283696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG562650.069.6195314
TGAGGTA1084800.069.594991
GATAGGC270200.069.4549515
GGTAGTA1208550.069.451874
GTTGTAT547050.069.44212
GTAGGTT743200.069.440418
TATTGGC360850.069.4364315
AGGTAGT1205700.069.429483
GATAGGT157350.069.4206615
AGTAGGT745050.069.380737
CGTAAAT352450.069.3641749
GTATGGT267950.069.3458915
GAGGTAG1232350.069.331012
GCAGCAC414700.069.3068853
CAGGATA581550.069.3004912
CAGCACG351400.069.299434
AGCTTAT160300.069.294942
TAGTAGG746400.069.294436
GTAGTAG1209200.069.25825
TAGGTTG744950.069.24339
Produced by FastQC (version 0.11.5)