FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA260_DODI1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA260_DODI1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064592
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG347700.38358041928417735No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT237280.2617657805227196No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG205780.22701518170922638No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC156980.1731793333886401No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA149750.16520324356573357No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT148990.16436481641975723No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA147910.1631733673175803No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC130120.1435475529400551No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT120990.13347539525220772No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1090950.069.627691
GGATAGG571550.069.6250514
GATAGGT147900.069.59630615
AGTAGGT777800.069.4425357
AGGTAGT1237600.069.4174353
GATAGGC281750.069.4147615
GGTAGTA1241000.069.4033054
GTAGGTT778100.069.3752758
TAGTAGG779100.069.371226
GAGGTAG1266850.069.331422
GTTGTAT568350.069.2961912
AAGTAAT592300.069.285364
CAGGATA589700.069.2741312
TTCAAGT572700.069.270211
GTAGTAG1242100.069.2431955
GTATGGT269900.069.23350515
TATAGTT414650.069.2304916
GGTACGC256450.069.2070314
TAGGTTG779700.069.178289
CAGCACG341100.069.1766744
Produced by FastQC (version 0.11.5)