FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA258_DOP1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA258_DOP1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11461443
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA709310.6188662282750959No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA698660.6095742045744152No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT437080.38134814263788597No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG315060.27488685325224754No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC281960.24600741808862986No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC258690.2257045644252648No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT167710.1463253797972908No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA121810.10627806638308981No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA121530.10603376904635829No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG116630.10175856565355688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1146450.069.6721041
GGATAGG551550.069.4476714
AGTAGGT758650.069.423987
CAGCACG580450.069.416464
GTTGTAT556900.069.41405512
CGTAAAT584300.069.3944559
GGTAGTA1269750.069.38824
GTAGGTT758600.069.386738
AGGTAGT1267400.069.378443
GCAGCAC655700.069.3732453
GAGGTAG1289350.069.3585742
TGGTACG262100.069.3442113
TAGTAGG760800.069.287936
GTATGGT264200.069.2578915
TATTGGC582950.069.25526415
TAGGTTG759450.069.252869
TTGGCGA456250.069.24766517
GGTACGC257850.069.23962414
TAGCAGC633600.069.227041
TTGGTAC262950.069.2265312
Produced by FastQC (version 0.11.5)