FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA248_DOM1_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA248_DOM1_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11195245
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG338420.3022890521824221No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA221940.1982448798574752No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC208680.1864005655972692No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA194930.17411856551598467No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT182420.16294417853293966No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT166080.14834869625452593No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG122050.10901949890332904No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1295150.069.607021
GGATAGG677750.069.57970414
GATAGGC329600.069.4458215
CGTAAAT414250.069.381859
AGGTAGT1428000.069.3812563
AGTAGGT880600.069.358737
GGTAGTA1432050.069.354054
GTAGGTT880600.069.34688
GTTGTAT642200.069.30585512
GAGGTAG1456550.069.287772
CAGGATA701350.069.2694212
GATAGGT180550.069.2419915
TATTGGC424100.069.2387615
TAGTAGG883100.069.199616
AGGATAG701350.069.15932513
CAGCACG412350.069.149394
GTAGTAG1433150.069.149355
TAGGTTG883250.069.146669
GTATGGT315250.069.13203415
GCAGCAC482900.069.131853
Produced by FastQC (version 0.11.5)