FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DJA247_DOP2_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DJA247_DOP2_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10797630
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA460710.426676965222924No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC439090.4066540527875098No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA437650.405320426797362No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG363460.3366109044299536No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC310340.2874149234600556No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT204110.1890322228118578No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT172090.15937756711426487No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACAACTGTAGGCACCA168590.1561361150548778No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA122240.11321002849699424No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTCAACTGTAGGCACCA120790.11186714121524816No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG114100.10567133713601966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1115400.069.594171
GGATAGG570450.069.5932614
AGGTAGT1231600.069.391643
GGTAGTA1234600.069.385624
GTAGGTT753500.069.3424768
GATAGGT160800.069.3234615
GTTGTAT556200.069.2874612
GATAGGC272950.069.28013615
GCAGCAC458000.069.267913
CAGGATA591400.069.2646812
AGTAGGT754700.069.264687
TATTGGC409850.069.2467115
GAGGTAG1256700.069.241282
CGTAAAT405250.069.232449
GTAGTAG1235050.069.187755
CAGCACG403400.069.1761554
TAGTAGG755400.069.15666
AGTAATC428600.069.1442955
AAGTAAT595550.069.112694
CCAGGAT425700.069.1109111
Produced by FastQC (version 0.11.5)