FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4601_AM4_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4601_AM4_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10844222
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA359930.33190947215945965No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA340170.3136877869154652No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG330620.3048812538142432No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC270860.24977356605204137No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT188450.17377917936390458No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT168240.1551425265915803No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC123090.11350745124915368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1295450.069.6177141
GGATAGG703850.069.6104414
GATAGGT186100.069.4905315
GGTAGTA1426450.069.448084
GTTGTAT632300.069.44446612
AGGTAGT1424400.069.439243
GTAGGTT873550.069.435638
CAGGATA726900.069.42020412
AGTAGGT874500.069.416557
TTCAAGT712800.069.408661
GAGGTAG1450450.069.386262
GATAGGC354250.069.3857615
TATTGGC468800.069.37865415
CAGCACG457850.069.3776254
AAGTAAT731150.069.373514
TAGTAGG876350.069.345116
GCAGCAC537700.069.3291243
GTAGATT348600.069.267638
CCAGGAT534650.069.25204511
GTAGTAG1427400.069.247655
Produced by FastQC (version 0.11.5)