FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4599_ADI5_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4599_ADI5_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10803603
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC385700.3570105269510551No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA304070.28145240064819116No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG304070.28145240064819116No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA222760.20619047182685257No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT156740.14508122891964836No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC153830.14238768307202698No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1167850.069.5956341
GGATAGG565750.069.57310514
AGTAGGT780500.069.4986957
GATAGGC266450.069.4614115
GTAGGTT780850.069.45418
GTTGTAT577100.069.4295612
AGGTAGT1285800.069.4037253
CGTAAAT513700.069.387749
GGTAGTA1289900.069.365274
TAGTAGG782400.069.36136
GAGGTAG1310250.069.320632
GATAGGT164700.069.31995415
TTGGCGA406000.069.3013817
TAGGTTG782250.069.2949
GGTACGC278700.069.2841514
TTCAAGT572050.069.284071
TATTGGC520950.069.281115
GCAGCAC588400.069.263953
TGGTACG284200.069.248113
AAGTAAT587700.069.245514
Produced by FastQC (version 0.11.5)