FastQCFastQC Report
Sat 14 Mar 2020
HCCCVBGXF_n01_DG4597_AP5_miRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCCCVBGXF_n01_DG4597_AP5_miRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10967877
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCAACTGTAGGCACCATC351510.32049046501889106No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA350890.3199251778625891No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG321370.29301021519479115No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA293530.2676269983698759No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT193260.17620547714019769No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT167480.15270047247976978No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC138660.12642373724650632No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTG120540.10990276422684171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGTA1224750.069.610511
GGATAGG660250.069.5688414
GTAGGTT820000.069.457748
GATAGGC322350.069.4227715
AGTAGGT820850.069.419927
GTTGTAT599250.069.4086412
GGTAGTA1353650.069.398144
AGGTAGT1350550.069.386023
CGTAAAT510550.069.3821959
TATTGGC518400.069.3703415
CAGGATA682550.069.3524412
GCAGCAC583850.069.334133
TAGTAGG821100.069.333616
GAGGTAG1376550.069.3309862
CAGCACG508100.069.32814
TTCAAGT666900.069.3029251
AAGTAAT684050.069.2972644
GATAGGT182750.069.2704615
TTGGCGA406450.069.25767517
TAGGTTG822250.069.245769
Produced by FastQC (version 0.11.5)